WebFITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate - has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes. ... Because of its broad absorption range and the fact that its emission spectrum is equivalent to APC, PE-Cy5 is not recommended for simultaneous use ... WebFluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
Fluorescein isothiocyanate (FITC) AAT Bioquest
Webabsorption of a photon of energy by a fluorophore, which occurs due to an interaction of the oscillating electric field vector of the light wave with charges (electrons) in the … WebTo only list those, select '2-Photon Cross-Sections' from the drop down menu. Unfortunately, also all one-photon spectra for the same dyes are shown in the list, so you may have to search a bit. The 2P-spectrum is sometimes included with the one-photon excitation (e.g. Cy5, Cy5.5). Usually, however, the two photon spectrum is indicated with -2P ... cheap holidays for 2022 all inclusive
Spectra Viewer Chroma Technology Corp
WebFluorescein Iso-thiocyanate (FITC) 495 519 488 EviTag TM quantum dots-Adirondack Green 505 520 ... Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. A tandem dye consists of a WebAlexa Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission spectra comparable to Fluorescein (FITC), but are much brighter, far more photostable and less sensitive to pH changes between pH 4 and 10. Alexa Fluor 488 conjugates are compatible with common fluorescein equipment, settings, or filters and are ideally suitable for all ... Web1. Prepare stock solutions of fluorescein and 1.0 M KI in water. The fluorescence of fluorescein is dependent on OH – concentration so the fluorescein stock solution is made up in a buffer solution that maintains constant [OH –] (that is constant pH). 2. Determine the absorption spectrum of fluorescein in water and I – solution to look ... cheap holidays for april 2023