WebApr 14, 2024 · The GO analysis (p < 0.01) suggested that the cis- and trans-target genes of the DE lncRNAs were primarily involved in the regulation of single organism processes, bone development, and bone maturation . The KEGG pathway analysis indicated that the target genes were involved in Wnt, TGF-beta, and Notch signaling pathways . WebI am trying to check for overexpression of a 38kd flag tagged protein in hela cells. I am unable to detect the protein of interest as flag antibody gives too many non specific bands in the 30-50 ...
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WebApr 10, 2024 · Ad-APN-GFP continuous expression and massive amplification in vitro. After Ad-APN-GFP was successfully constructed, a titer of 1 × 10 11 was obtained via purification and amplification. The average number of positive cells in five fields under the microscope at 10 7 times dilution was 13 (Fig. 2A), and a virus titer of 1.02 × 10 11 (PFU /ml) was … WebApr 5, 2024 · The transcription analysis of the N-hydroxylase-encoding xiaH by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that the transcription level of xiaH responsible for the formation of a nitroxyl radical was increased by the overexpression of orf2011, which is located outside the xiamycin biosynthetic gene … hairdressers front st chester le street
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