2024 Oxidized ldl uptake assay kit

Oxidized ldl uptake assay kit

Author: cbtq

August undefined, 2024

Webating many kinds of chemicals. This assay, based on the LDL (low density lipoprotein)-uptake activity of human hepatoblastoma cells, Hep G2, can evaluate cytotoxicity for 48 h with high sensitivity and selectiv-ity using a 96 well plate and a fluorescent plate reader. We evaluate the toxicity of 230 kinds of chemicals and WebAssay kit format. Sandwich ELISA Kit. Detector antibody conjugate. Biotin. Label or dye. HRP . Gene aliases. CLEC8A, LOX1, LOXIN, OLR1, SCARE1, SLOX1. Gene ID (Human) 4973. Gene symbol. ... LOX-1 (lectin-like oxidized LDL receptor -1) is a 40-50 kDa type II membrane glycoprotein class E scavenger receptor expressed by endothelial cells ...

Oxidized LDL Uptake Assay Kit - Bertin Bioreagent

WebLipid Peroxidation Oxidized LDL ELISA Kits LDL cholesterol, sometimes referred to as “bad” cholesterol, is even more dangerous when it becomes oxidized. Oxidized LDL ( OxLDL) is … Web· 7-AAD is included in LDL Uptake Flow Cytometry Assay Kit and Oxidized LDL Uptake Assay Kit for live-cell gating LDL uptake in Huh7 human hepatocytes. Huh7 cells were plated at 1 x 104 cells/well in a 96 well plate and allowed to attach and grow for 24 hours. LDL-DylightTM 550 was added in serum-free medium at a show me 50 states

Human Oxidized Low Density Lipoprotein (Human Ox-LDL) 人源氧 …

WebDay 2: Add drug modifying LDL uptake, incubate 4 hours. Add BODIPY-LDL. Incubate overnight. Day 3: Wash wells with PBS, add PBS to avoid cell drying, and read uptake on fluorimeter. All ... WebJul 31, 2024 · Oxidized LDL stimulation increases production of macrophage prothrombotic microparticles. ELISA measurement of TF content in washed microparticles from resting and oxLDL‐treated (25 µg/mL) macrophages (A). Impedance flow cytometry (iFACS) analysis of TF‐positive MΦMPs (B, E). Quantification of TF‐positive MΦMP events … WebNov 18, 2014 · LDL uptake assay protocol summary: - remove culture medium from experimentally treated cells. - add LDL-Dylight 550 solution and incubate for 3-4 hrs. - … show me 60 degree angle

A New Assay for Evaluating Hepatotoxicity and Cytotoxicity …

Vimentin is an endogenous ligand for the pattern recognition …

WebCompare Oxidized LDL Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Web2.1. Purification and Oxidation of LDL. μm), and stored at 4°C. The protein concentration was determined by the BCA kit (Pierce, USA). Oxidized LDL (oxLDL) was obtained by incubation of LDL (2 mg/mL) with 20 μM CuSO 4 for 18 h at 37°C. The oxidation of both protocols was terminated by the addition of 0.5 mM EDTA. 2.2. Human THP-1 Monocytic ... show me 7/8 inch on a rulerWebOxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological … show me 726 lionel engines for sale

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Oxidized ldl uptake assay kit

Human Oxidized Low Density Lipoprotein (Human Ox-LDL) 人源氧 …

WebOxLDL ELISA Kits. OxLDL ELISA kits are assays designed to detect oxidized low-density lipoprotein, a key protein complex involved in lipid transport and metabolism in humans. It serves as a ligand for the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) which is expressed in macrophages, smooth muscles and endothelial cells. WebNov 18, 2015 · Mol-2 and Mol-3 had no significant effect on ox-LDL uptake (P > 0.05). Overall, Mol-5 exhibited the maximum inhibitory effect on ox-LDL uptake by HUVECs . We also measured the dose response to ...

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WebThe kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 … WebCayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells.

WebHUFI00713. Size: 96 Assays. Alias: OxLDL, Oxidized low-density lipoprotein. Detection method: Sandwich ELISA, Double Antibody. Application: This immunoassay kit allows for the in vitro quantitative determination of Human OxLDL concentrations in serum plasma and other biological fluids. Web7. STA-389: Human Oxidized LDL ELISA Kit (HNE-LDL Quantitation) ... 9. STA-391: HDL and LDL/VLDL Cholesterol Assay Kit Kit Components 1. Dextran Solution (Part No. 260801): One 8 mL bottle 2. ... Protein deglycosylation can drastically effect the cellular uptake. Nanoscale. doi: 10.1039/C8NR08305C. 7. Frank, A.C. et al. (2024). Apoptotic tumor ...

WebOxidation of LDL is natural process within the body, and oxidized LDL complexed with DiI dye (DiI-OxLDL) is an important tool in the study of scavenger receptor-mediated endocytosis by macrophages and … WebIn atherosclerotic lesions, MDM are the primary source of NADPH oxidase-derived superoxide anion (O2-) inducing low-density lipoprotein (LDL) oxidation leading to their unregulated uptake of oxidized LDL and foam cell formation. We recently discovered that zymosan potently activates monocyte NADPH oxidase via the non-toll pattern recognition ...

WebApr 12, 2024 · A glucose uptake assay was conducted in insulin resistance HepG2 (IR-HepG2) cells to establish the antidiabetic activity of the Lc-EAF. ... for biochemical analysis. The levels of glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT ...

WebCat No: 601180. Assay Kits - Cell-Based Assays. The oxidation of low density lipoprotein is thought to be one of the first steps in atherosclerosis. The uptake of oxidized LDL … show me 70mmWebBCA蛋白浓度测定试剂盒 (增强型) (Enhanced BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。. 和碧云天生产的普通BCA蛋白浓度测定试剂盒相比，灵敏度更高，检 … show me 7 centimetersWebAug 21, 2024 · The aim of this in vitro study was to examine the effect of raspberry polyphenolic extract on the immune-metabolic molecular mechanisms activated by obesity-related signals in hepatocytes (HB-8965®). Alterations in endosomal/lysosomal activity (neutral red uptake assay, NR), the expression of selected genes involved with lipid … show me 7/8 on rulerWebOxidized Low-density Lipoprotein (oxLDL) oxLDL is a major component of atherosclerotic lesions, and T lymphocytes reactive to oxLDL have been recovered from human atherosclerotic plaques ( Stemme et al., 1995 ). It seems likely that the oxidation of oxLDL renders the molecule non-self, thus initiating an autoimmune response. show me 7/32 on a rulerWebDetect oxidized LDL uptake by cultured cells by flow cytometry or fluorescence microscopy. Flow-based readout allows possibility of multiplexing. Inhibitor of oxLDL uptake is … Cayman’s Oxidized LDL Uptake Assay Kit is a convenient tool to study modulators of … Register for Your Account . Manage your data, track shipments, review your order … We collect cookies for vital website function and to better serve our customers. By … show me 7/8 on a rulerWebThe LDL Uptake Assay Kit (Flow cytometry) (ab236208) employs human LDL conjugated to DyLight TM 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow … show me 7mm in inchesWebDocuments. FAQ. The Image-iT Low Density Lipoprotein Uptake Kit - pHrodo Red includes pHrodo Red dye-labeled LDL (also available separately), unlabeled LDL, heparin, BSA, and … show me 7mm