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Pulk1 s555

WebNov 1, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity. WebWestern blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 µM, 30 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb (left). …

Markers of autophagic activation analyzed in a cytosolic fraction ...

WebULK1 was similar between healthy and dystrophic muscles, however, pULK1 S555 was decreased by 0.5-fold (p < 0.05) in D2-mdx compared to DBA and the ratio was similar … WebThe FK506-binding protein 51 (FKBP51, encoded by Fkbp5) is the main modulator of the stress response and is best characterized as a co-chaperone to HSP90, thereby orchestrating diverse pathways important to maintain homeostatic control (14–17).We and others have provided evidence that FKBP51 is associated with type 2 diabetes and have … grafton swivel accent chair https://daniutou.com

Anti-ULK1 (phospho S555) antibody (ab229537) Abcam

WebJan 18, 2024 · Whole cell extracts from MEFs, ESCs, and iPSCs were harvested for western blot using anti-p-ULK1(S555), anti-p-ULK1(S757), and anti-ULK1 antibodies. ULK1 serves as a loading control. Web(A) ULK1, pULK1 S555, Beclin1and pBeclin1 S91 were decreased, while PI3K III was elevated in D2-mdx gastrocnemius cytoplasmic fraction (CF) compared to DBA. (B) … WebAug 14, 2015 · Although ULK1 phosphorylation at S555 (pULK1-S555), S777 (pULK1-S777) and S467 (pULK1-S467) was detected only in wild-type but not AMPK−/− cells … china eastern airlines accident

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Pulk1 s555

LCN2 deficiency ameliorates doxorubicin-induced …

WebSep 28, 2024 · Therefore, we overexpressed wild type BRSK2 in HEK293T cells and measured changes in pULK1 S317 and S555 following treatment with increasing doses of GW296115 for 2 h. WebJul 9, 2024 · Search worldwide, life-sciences literature Search. Advanced Search

Pulk1 s555

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WebOct 1, 2024 · Further, treatment with RAD001 was associated with a considerable increase in pULK1 S555 and ULK1 levels through the inhibition of mTORC1. 3‐MA reversed the … WebAnti-phospho ULK1 Antibody (Ser777) from rabbit, purified by affinity chromatography; Synonyms: Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase …

WebJul 13, 2024 · a–e Immunoblot analysis of LC3, SQSTM1, AMPK phosphorylated in T172 (pAMPK) and ULK1 phosphorylated in S555 (pULK1) in U2OS cells treated 24 h with three increasing doses of DCAP (1, 5 and 10 ... WebJan 15, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity.

WebNational Center for Biotechnology Information WebAug 14, 2015 · Although ULK1 phosphorylation at S555 (pULK1-S555), S777 (pULK1-S777) and S467 (pULK1-S467) was detected only in wild-type but not AMPK−/− cells (Supplementary Fig. 1A,L), pULK was not ...

WebCell Signaling Technology Inc rabbit monoclonal anti pulk1 s555 Rabbit Monoclonal Anti Pulk1 S555, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz …

WebBackground Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene unc-51 in … grafton swimming poolWebJan 15, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess … china eastern airlines a320WebMar 1, 2024 · WT and Ulk1/2 −/− MEFs were treated with dsDNA (4 μg/ml) for the indicated time interval, and lysates were immunoblotted for pULK1 S555, ULK, STING, and β‐actin. J Control, cGAS KO, and STING KO THP1 cells were treated with dsDNA (4 μg/ml) for the indicated time interval, and lysates were immunoblotted for cGAS, STING, pTBK1, … grafton tabb football and cheerWebAll lanes : Anti-ULK1 (phospho S555) antibody (ab229537) at 1/1000 dilution Lane 1 : Untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract Lane 2 : … grafton swivel chairWebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin (used as loading control) with specific antibodies; H:ULK1-to-β-actin;I: pULK1-s757-to β-actin;J: pULK1-s555-to β-actin;K: pULK1-s317-toβ-actin.All proteins were normalized to β-actin. china eastern airlines callWebAlphaLISA SureFire Ultra Human Phospho-ULK1 (Ser556) Detection Kit, 500 Assay Points PerkinElmer. Life Sciences & Diagnostics. Products & Services. Life Sciences Products. Drug Discovery Reagents. Alpha Assays. china eastern airlines book flightschina eastern airlines boeing 737 800