WebNov 1, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity. WebWestern blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 µM, 30 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb (left). …
Markers of autophagic activation analyzed in a cytosolic fraction ...
WebULK1 was similar between healthy and dystrophic muscles, however, pULK1 S555 was decreased by 0.5-fold (p < 0.05) in D2-mdx compared to DBA and the ratio was similar … WebThe FK506-binding protein 51 (FKBP51, encoded by Fkbp5) is the main modulator of the stress response and is best characterized as a co-chaperone to HSP90, thereby orchestrating diverse pathways important to maintain homeostatic control (14–17).We and others have provided evidence that FKBP51 is associated with type 2 diabetes and have … grafton swivel accent chair
Anti-ULK1 (phospho S555) antibody (ab229537) Abcam
WebJan 18, 2024 · Whole cell extracts from MEFs, ESCs, and iPSCs were harvested for western blot using anti-p-ULK1(S555), anti-p-ULK1(S757), and anti-ULK1 antibodies. ULK1 serves as a loading control. Web(A) ULK1, pULK1 S555, Beclin1and pBeclin1 S91 were decreased, while PI3K III was elevated in D2-mdx gastrocnemius cytoplasmic fraction (CF) compared to DBA. (B) … WebAug 14, 2015 · Although ULK1 phosphorylation at S555 (pULK1-S555), S777 (pULK1-S777) and S467 (pULK1-S467) was detected only in wild-type but not AMPK−/− cells … china eastern airlines accident